This novel hyphenated system accomplishes all of the following functions in a single run:

- Detection and quantitation of ~ 10 CPM peaks on-line
– Acquisition of mass spectra
– Automatic isolation of metabolites based on UV or radioactivity for further analysis

System Configuration

LCs: Agilent 1100, Shimadzu, Alliance 2695, or PerkinElmer S200
StopFlow controller (Model A or B)
Radioactivity detectors: Packard 150, 500, IN/US Beta-RAM, Raytest Ramona, or Berthold LB509
Optional: Fraction collectors: Isco fraction collectors: Foxy® Jr., Foxy 200, Gilson FC204 or XL222
Optional: Mass spectrometers: Finnigan XCalibur 1.2 or above, Quantum, Micromass MS or Sciex MS, etc.

Please see Compatibility with Existing Radio-LC (PDF file).

Applications:

Metabolite Identification with LC-ARC-MS-FC systems

On-Line Liquid Chromatography-Accurate Radioisotope Counting Coupled with a Radioactivity Detector and Mass Spectrometer for Metabolite Identification in Drug Discovery and Development

A Novel Hyphenated LC/ARC/RD/MS/FC System for Identification of Drug Metabolites